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gexscope tissue preservation solution  (Singleron Biotechnologies)

 
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    Singleron Biotechnologies gexscope tissue preservation solution
    Gexscope Tissue Preservation Solution, supplied by Singleron Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gexscope+tissue+preservation+solution/gexscope+preservation+solution+tissue/10__1097_slash_hc9__0000000000000973-86-15-19
    Average 86 stars, based on 1 article reviews
    gexscope tissue preservation solution - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: AAV gene therapy for homozygous familial hypercholesterolemia: a phase 1 trial.
    Article Snippet: Homozygous familial hypercholesterolemia (HoFH) is a rare autosomal disease characterized by severely elevated low-density lipoprotein cholesterol (LDL-C) levels and accelerated atherosclerotic cardiovascular disease.. More than 80% of patients with HoFH carry low-density lipoprotein receptor (LDLR) mutations.. Here we developed an adeno-associated virus gene therapy designed to express LDLR in the liver and evaluated its safety and efficacy in lowering LDL-C levels in experimental animals and in patients with HoFH.

    Concentration Assay:

    Article Title: AAV gene therapy for homozygous familial hypercholesterolemia: a phase 1 trial.
    Article Snippet: Homozygous familial hypercholesterolemia (HoFH) is a rare autosomal disease characterized by severely elevated low-density lipoprotein cholesterol (LDL-C) levels and accelerated atherosclerotic cardiovascular disease.. More than 80% of patients with HoFH carry low-density lipoprotein receptor (LDLR) mutations.. Here we developed an adeno-associated virus gene therapy designed to express LDLR in the liver and evaluated its safety and efficacy in lowering LDL-C levels in experimental animals and in patients with HoFH.

    Preserving:

    Article Title: AAV gene therapy for homozygous familial hypercholesterolemia: a phase 1 trial.
    Article Snippet: Homozygous familial hypercholesterolemia (HoFH) is a rare autosomal disease characterized by severely elevated low-density lipoprotein cholesterol (LDL-C) levels and accelerated atherosclerotic cardiovascular disease.. More than 80% of patients with HoFH carry low-density lipoprotein receptor (LDLR) mutations.. Here we developed an adeno-associated virus gene therapy designed to express LDLR in the liver and evaluated its safety and efficacy in lowering LDL-C levels in experimental animals and in patients with HoFH.

    Article Title: Cannabidiol suppresses emergency MDSCs generation by disturbing EEF1B2-mediated C/EBP β protein synthesis in colorectal adenomas.
    Article Snippet: .. Mice colon collection and processing for single-cell RNA sequencing Freshly dissected colon tissues were initially rinsed with PBS, then transferred into GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies) to maintain cell viability, and stored on ice. ..

    Article Title: Cannabidiol suppresses emergency MDSCs generation by disturbing EEF1B2-mediated C/EBPβ protein synthesis in colorectal adenomas
    Article Snippet: .. Freshly dissected colon tissues were initially rinsed with PBS, then transferred into GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies) to maintain cell viability, and stored on ice. ..

    Article Title: Immunosuppressive immune microenvironment landscapes in VISTA-high gastric cancer
    Article Snippet: .. Fresh tumour tissues were preserved in GEXSCOPE® Tissue Preservation Solution (Singleron Biotechnologies, Nanjing, China) and transported on ice to the laboratory. .. After washing with Hanks’ Balanced Salt Solution (HBSS), tissues were minced into 1–2 mm pieces, digested with GEXSCOPE ® Tissue Dissociation Solution (Singleron Biotechnologies) at 37 °C for 15 minutes, and filtered through 40-μm strainers.

    Article Title: Depletion of NAT10 in T cells attenuates metabolic dysfunction–associated steatohepatitis in mice
    Article Snippet: .. Single-cell RNA sequencing sample preparation and cell isolation Fresh liver specimens were immediately immersed in GEXSCOPE tissue preservation solution (Singleron Biotechnologies, Nanjing, China) and maintained on ice for processing within 30 minutes post-resection. .. Briefly, tissues were washed 3 times with ice-cold Hank’s Balanced Salt Solution (HBSS; Gibco), mechanically minced into 1–2 mm3 fragments using sterile scalpels, and enzymatically digested in 2 mL of GEXSCOPE tissue dissociation solution (Singleton) at 37 °C for 15 minutes with continuous orbital shaking (200 rpm).

    Article Title: Multi-omics integration defines an ECM-associated intratumoral heterogeneity signature enabling prognosis assessment and therapeutic stratification in hepatocellular carcinoma.
    Article Snippet: Intratumoral heterogeneity (ITH) is a fundamental driver of hepatocellular carcinoma (HCC) progression and therapeutic resistance.. Here, we developed a multi-omics-derived ITH scoring system to improve patient stratification and guide precision therapy.. Using somatic mutation data from 361 TCGA HCC patients, we quantified genomic heterogeneity via the Mutant-Allele Tumor Heterogeneity (MATH) score, while single-cell RNA sequencing (scRNA-seq) of 20 HCC samples (our cohort and GSE149614) enabled assessment of cellular diversity.

    Article Title: Germ layer specification and organotropism in lymphoma invasion.
    Article Snippet: .. Following digestion with GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies, USA) for 15 min at 37 °C, cells were collected by filtration through a 40-lm sterile strainer (Corning, USA) to separate them from cellular debris and impurities. .. After a 10-min incubation with 2 mL of GEXSCOPE Red Blood Cell Lysis Buffer (Singleron Biotechnologies, USA) at 25 °C, the cell suspension was centrifuged (500 g, 5 min); the pellet was then resuspended in PBS prior to cell counting on a TC20 automated cell counter (Bio-Rad, USA).

    Article Title: Immunosuppressive immune microenvironment landscapes in VISTA-high gastric cancer.
    Article Snippet: .. Two metrics, effective score (immune cell-to-tumour cell ratio) and effective percentage (proportion of tumour cells paired with immune cells), were used to evaluate these networks: Effective score 1⁄4 number of paired immune cells number of central tumor cells Effective percent 1⁄4 number of central tumor cells with paired immune cells number of central tumor cells These spatial dynamics offer insights into cell functions in the TME, which is crucial for understanding ligand-receptor-mediated interactions that influence antitumor or immunosuppressive effects. scRNA-seq sample collection and preparation Fresh tumour tissues were preserved in GEXSCOPE® Tissue Preservation Solution (Singleron Biotechnologies, Nanjing, China) and transported on ice to the laboratory. .. After washing with Hanks’ Balanced Salt Solution (HBSS), tissues were minced into 1–2mm pieces, digested with GEXSCOPE® Tissue Dissociation Solution (Singleron Biotechnologies) at 37 °C for 15minutes, and filtered through 40-μm strainers.

    Mouse Assay:

    Article Title: Cannabidiol suppresses emergency MDSCs generation by disturbing EEF1B2-mediated C/EBP β protein synthesis in colorectal adenomas.
    Article Snippet: .. Mice colon collection and processing for single-cell RNA sequencing Freshly dissected colon tissues were initially rinsed with PBS, then transferred into GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies) to maintain cell viability, and stored on ice. ..

    Single Cell:

    Article Title: Cannabidiol suppresses emergency MDSCs generation by disturbing EEF1B2-mediated C/EBP β protein synthesis in colorectal adenomas.
    Article Snippet: .. Mice colon collection and processing for single-cell RNA sequencing Freshly dissected colon tissues were initially rinsed with PBS, then transferred into GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies) to maintain cell viability, and stored on ice. ..

    Article Title: Depletion of NAT10 in T cells attenuates metabolic dysfunction–associated steatohepatitis in mice
    Article Snippet: .. Single-cell RNA sequencing sample preparation and cell isolation Fresh liver specimens were immediately immersed in GEXSCOPE tissue preservation solution (Singleron Biotechnologies, Nanjing, China) and maintained on ice for processing within 30 minutes post-resection. .. Briefly, tissues were washed 3 times with ice-cold Hank’s Balanced Salt Solution (HBSS; Gibco), mechanically minced into 1–2 mm3 fragments using sterile scalpels, and enzymatically digested in 2 mL of GEXSCOPE tissue dissociation solution (Singleton) at 37 °C for 15 minutes with continuous orbital shaking (200 rpm).

    RNA Sequencing:

    Article Title: Cannabidiol suppresses emergency MDSCs generation by disturbing EEF1B2-mediated C/EBP β protein synthesis in colorectal adenomas.
    Article Snippet: .. Mice colon collection and processing for single-cell RNA sequencing Freshly dissected colon tissues were initially rinsed with PBS, then transferred into GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies) to maintain cell viability, and stored on ice. ..

    Article Title: Depletion of NAT10 in T cells attenuates metabolic dysfunction–associated steatohepatitis in mice
    Article Snippet: .. Single-cell RNA sequencing sample preparation and cell isolation Fresh liver specimens were immediately immersed in GEXSCOPE tissue preservation solution (Singleron Biotechnologies, Nanjing, China) and maintained on ice for processing within 30 minutes post-resection. .. Briefly, tissues were washed 3 times with ice-cold Hank’s Balanced Salt Solution (HBSS; Gibco), mechanically minced into 1–2 mm3 fragments using sterile scalpels, and enzymatically digested in 2 mL of GEXSCOPE tissue dissociation solution (Singleton) at 37 °C for 15 minutes with continuous orbital shaking (200 rpm).

    Sample Prep:

    Article Title: Depletion of NAT10 in T cells attenuates metabolic dysfunction–associated steatohepatitis in mice
    Article Snippet: .. Single-cell RNA sequencing sample preparation and cell isolation Fresh liver specimens were immediately immersed in GEXSCOPE tissue preservation solution (Singleron Biotechnologies, Nanjing, China) and maintained on ice for processing within 30 minutes post-resection. .. Briefly, tissues were washed 3 times with ice-cold Hank’s Balanced Salt Solution (HBSS; Gibco), mechanically minced into 1–2 mm3 fragments using sterile scalpels, and enzymatically digested in 2 mL of GEXSCOPE tissue dissociation solution (Singleton) at 37 °C for 15 minutes with continuous orbital shaking (200 rpm).

    Cell Isolation:

    Article Title: Depletion of NAT10 in T cells attenuates metabolic dysfunction–associated steatohepatitis in mice
    Article Snippet: .. Single-cell RNA sequencing sample preparation and cell isolation Fresh liver specimens were immediately immersed in GEXSCOPE tissue preservation solution (Singleron Biotechnologies, Nanjing, China) and maintained on ice for processing within 30 minutes post-resection. .. Briefly, tissues were washed 3 times with ice-cold Hank’s Balanced Salt Solution (HBSS; Gibco), mechanically minced into 1–2 mm3 fragments using sterile scalpels, and enzymatically digested in 2 mL of GEXSCOPE tissue dissociation solution (Singleton) at 37 °C for 15 minutes with continuous orbital shaking (200 rpm).

    Filtration:

    Article Title: Germ layer specification and organotropism in lymphoma invasion.
    Article Snippet: .. Following digestion with GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies, USA) for 15 min at 37 °C, cells were collected by filtration through a 40-lm sterile strainer (Corning, USA) to separate them from cellular debris and impurities. .. After a 10-min incubation with 2 mL of GEXSCOPE Red Blood Cell Lysis Buffer (Singleron Biotechnologies, USA) at 25 °C, the cell suspension was centrifuged (500 g, 5 min); the pellet was then resuspended in PBS prior to cell counting on a TC20 automated cell counter (Bio-Rad, USA).

    Sterility:

    Article Title: Germ layer specification and organotropism in lymphoma invasion.
    Article Snippet: .. Following digestion with GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies, USA) for 15 min at 37 °C, cells were collected by filtration through a 40-lm sterile strainer (Corning, USA) to separate them from cellular debris and impurities. .. After a 10-min incubation with 2 mL of GEXSCOPE Red Blood Cell Lysis Buffer (Singleron Biotechnologies, USA) at 25 °C, the cell suspension was centrifuged (500 g, 5 min); the pellet was then resuspended in PBS prior to cell counting on a TC20 automated cell counter (Bio-Rad, USA).



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